Type of device

Confocal laser scanning microscope by Zeiss

Short description

The Zeiss LSM980 with Airyscan 2 is optimized for the simultaneous spectral detection of multiple weak labels with highest light efficiency. It offers excellent imaging performance combined with low phototoxicity and high speed. It can be used for multi-color experiments with living or fixed samples with highest sensitivity and spectral flexibility.

Instrument parameters

Technical specifications:	Inverse microscope LSM 980 Incubator XLmulti S1 with temperature control Optional lid for CO2 and O2 control z-piezo LED Colibri 7 Definite Focus 2 T-PMT detector for transmission Airyscan 2 with 32 GaAsP-detector elements for Super-resolution imaging
Objectives:	Plan-Apochromat 10x/0,45 M27 Plan-Apochromat 20x/0,8 M27 Plan-Apochromat 40x/0,95 Korr M27 LD LCI Plan-Apochromat 40x/1,2 Imm Korr DIC M27 Plan-Apochromat 63x/1,4 Oil DIC M27
Laser:	Dioden Laser 405 nm Dioden Laser 488 nm DPSS Laser 561 nm Dioden Laser 639 nm
Software:	ZEN blue

Methods & Expertise

Tiles, z-stack, time series, bleaching, FRAP

Reference publications

Komaragiri, Y. , Pires, R. H., Spiegler, S, Dau, H. T., Biedenweg, D., Salas, C. O., Hossain, Md. F., Fregin, B., Gross, S., Gellert, M., Lendeckel, U., Lillig, C. & Otto, O. (2024) Reactive oxygen species induce cell stiffening through lysosomal degradation and subsequent intracellular acidosis in myeloid precursor cells. Communications Physics 7:252

Affiliation, Contact & Terms of use

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Biosafety level:	S1