Leica TCS SP5II

Type of device

Confocal laser scanning microscope by Leica

Short description

The Leica TCS SP5II enables the user to examine the specimens in different modes:

• bright field
• Förster resonance energy transfer (FRET)
• Fluorescence recovery after photobleaching (FRAP)

Instrument parameters

Technical specifications:	Inverse research microscope DMI 6000 CS Motorized objective nosepiece XY motorized stage DMI 6000 SuperZ Galvo Stage Type H AOBS, 8 channel-AOTF
Objectives:	HC PL FLOUTAR 5x/0,15 HC PL APO 10x/0,40 CS HC PL APO 20x/0,7 IMM CORR CS HCX PL APO 63x/1,30 GLYC CORR 37°C
Laser:	Argon laser 458 nm + 476 nm + 488 nm + 498 nm + 514 nm Dioden laser, 405 nm HeNE laser, 633 nm + 594 nm DPSS laser, 561 nm
Software:	LAS Leica Application Software

No expertise regarding

FRET, FRAP, 2 Photon Microscopy

Reference publications

Kümmerlen, K., Raspe, S. & Harzsch, S. (2023) Neurochemical diversity in the central olfactory pathway of the crustacean Parhyale hawaiensis (Amphipoda): evolutionary implications. Journal of Comparative Neurology 531(10): 1032-1056

Affiliation, Contact & Terms of use

Affiliation:	University of Greifswald, Imaging Center of the Department of Biology
Location:	University of Greifswald Dept. of Cytology and Evolutionary Biology Soldmannstrasse 23 17498 Greifswald
Contact person:	Prof. Dr. Steffen Harzsch steffen.harzsch@uni-greifswald.de | Phone: 03834 420 4124
Terms of use:	https://imaging.uni-greifswald.de/en/general-terms-of-use/nutzungsordnungen/fluorescence-microscopy-at-the-zoological-institut-and-museum/
Biosafety level:	None