AxioImager.M2

Type of device

Fluorescence microscope by Zeiss

Short description

Modes:

• Bright field
• Fluorescence (DAPI, GFP, RFP, CFP, Cy5)

Instrument parameters

Technical specifications:	Upright system Motorized stage z-stacking HPX 120 C light source
Objectives:	EC PlnN 10x/0.3 Ph1 DICI EC PlnN 40x/0.75 Ph2 EC PlnN 100x/1,3 Oil Ph3
Camera:	AxioCam MRm3
Software:	Zen 2011

Methods & Expertise

Fluorescence in situ hybridization

Reference publications

Petersen, I., Schlüter, R., Hoff, K.J., Liebscher, V., Bange, G., Riedel, K. & Pané-Farré, J. (2020) Non-invasive and label-free 3D-visualization shows in vivo oligomerization of the staphylococcal alkaline shock protein 23 (Asp23). Sci Rep. 10(1):125.

Hinzke, T., Kleiner, M., Meister, M., Schlüter, R., Hentschker, C., Pané-Farré, J., Hildebrandt, P., Felbeck, H., Sievert, S.M., Bonn, F., Völker, U., Becher, D., Schweder, T. & Markert, S. (2021) Bacterial symbiont subpopulations have different roles in a deep-sea symbiosis. Elife 10:e58371

Affiliation, Contact & Terms of use

Affiliation:	University of Greifswald, Imaging Center of the Department of Biology
Location:	Institute of Microbiology Felix-Hausdorff-Str. 8
Contact person:	XXXX
Terms of use:	XXXX
Biosafety level:	S2