Operetta CLS

Type of device

High-Content Imager / Confocal Scanning Microscopy / Live-Cell Imaging
Operetta CLS by PerkinElmer (Revity)

Short description

The device is flexible for different imaging situations that require quantification:

• Well plate plate types and slides
• Automated re-imaging, e.g., re-image cells with 10% largest nuclei at higher magnification
• Widefield and spinning-disk confocal imaging
• Live-cell imaging (37°C, CO₂ variable) over time also with water-objectives (autom. refill)

Instrument parameters

Technical specifications:	sCMOS camera Motorized stage Confocal Disk Transmitted light and digital phase contrast Ratiometric FRET 3D-imaging and live cell imaging Fluorescence, fluorescence transmittance, transmission
Objectives:	3 air and 3 water-emmersion objectives permanently installed All objectives in the following table except 20x hNA air and 40x hNA air
Laser:	8 LEDs
Software:	Harmony 4.9

Methods & Expertise

Many different imaging assays and quantifications, e.g., migration assay, 3D spheroid growth tracking, organoid imaging and three-dimensional object segmentation and quantification, zebrafish larvae imaging, actin length quantification, scratch assays, single-cell sorting verification (single cell per well) > all in 96-well plates or similar; whole tissue section imaging on up to four slides, prescan-rescan assays for automated re-imaging of specific sub-populations within cell lines and primary cells

Reference publications

Over 10 publications together with University (Medicine) Greifswald, e.g., on human organoids (DOI: 10.1007/s00018-022-04355-6 Fig. 4e)
Over 30 publications in house, e.g., on high-throughput micronucleus analysis (DOI: 10.3390/nano12244463 Fig. 1), actin protrusion quantification (DOI: 10.1038/s41598-018-37169-3 Fig. 5), and whole-tissue imaging and quantification (DOI: 10.1080/2162402X.2024.2425124 Fig. 1-3)

Affiliation, Contact & Terms of use

Affiliation:	XXXX
Location:	XXXX
Contact person:	XXXX
Terms of use:	XXXX
Biosafety level:	None/S2