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Version vom 29. April 2025, 12:40 Uhr

Type of device

Fluorescence microscope by Zeiss

Short description

Modes:

Bright field
Fluorescence (DAPI, GFP, RFP, CFP, Cy5)

Instrument parameters

Technical specifications:	Upright system Motorized stage z-stacking HPX 120 C light source
Objectives:	EC PlnN 10x/0.3 Ph1 DICI EC PlnN 40x/0.75 Ph2 EC PlnN 100x/1,3 Oil Ph3
Camera:	AxioCam MRm3
Software:	Zen 2011

Methods & Expertise

Fluorescence in situ hybridization

Reference publications

Petersen, I., Schlüter, R., Hoff, K.J., Liebscher, V., Bange, G., Riedel, K. & Pané-Farré, J. (2020) Non-invasive and label-free 3D-visualization shows in vivo oligomerization of the staphylococcal alkaline shock protein 23 (Asp23). Sci Rep. 10(1):125.

Hinzke, T., Kleiner, M., Meister, M., Schlüter, R., Hentschker, C., Pané-Farré, J., Hildebrandt, P., Felbeck, H., Sievert, S.M., Bonn, F., Völker, U., Becher, D., Schweder, T. & Markert, S. (2021) Bacterial symbiont subpopulations have different roles in a deep-sea symbiosis. Elife 10:e58371

Affiliation, Contact & Terms of use

Affiliation:	XXXX
Location:	XXXX
Terms of use:	XXXX
Biosafety level:	S2

@@ Zeile 1: / Zeile 1: @@
 = Type of device =
-*Fluorescence microscope by Zeiss
+Fluorescence microscope by Zeiss
 = Short description =
-* Modes:
+Modes:
-**Bright field
+*Bright field
-**Fluorescence (DAPI, GFP, RFP, CFP, Cy5)
+*Fluorescence (DAPI, GFP, RFP, CFP, Cy5)
 = Instrument parameters =
-{| border="1"
+{| border="2"
-|Technical specifications: || Upright system
+|style="width:200px; text-align:left;" |Technical specifications:     ||style="width:300px; text-align:left;" |
+:Upright system
 :Motorized stage
 :z-stacking
 :HPX 120 C light source
 |-
-|Objectives: || EC PlnN 10x/0.3 Ph1 DICI, EC PlnN 40x/0.75 Ph2, EC PlnN 100x/1,3 Oil Ph3
+|Objectives: ||
+:EC PlnN 10x/0.3 Ph1 DICI
+:EC PlnN 40x/0.75 Ph2
+:EC PlnN 100x/1,3 Oil Ph3
 |-
-|Camera: || AxioCam MRm3
+|Camera: ||
+:AxioCam MRm3
+|-
+|Software: ||
+:Zen 2011
 |}
+= Methods & Expertise =
+Fluorescence ''in situ'' hybridization
-*Technical specifications:
-**Upright system
-**Motorized stage, z-stacking
-**HPX 120 C light source
-*Objectives:
+= Reference publications =
-**EC PlnN 10x/0.3 Ph1 DICI
+Petersen, I., Schlüter, R., Hoff, K.J., Liebscher, V., Bange, G., Riedel, K. & Pané-Farré, J. (2020) Non-invasive and label-free 3D-visualization shows ''in vivo'' oligomerization of the staphylococcal alkaline shock protein 23 (Asp23). Sci Rep. 10(1):125.
-**EC PlnN 40x/0.75 Ph2
-**EC PlnN 100x/1,3 Oil Ph3
-*Camera:
+Hinzke, T., Kleiner, M., Meister, M., Schlüter, R., Hentschker, C., Pané-Farré, J., Hildebrandt, P., Felbeck, H., Sievert, S.M., Bonn, F., Völker, U., Becher, D., Schweder, T. & Markert, S. (2021) Bacterial symbiont subpopulations have different roles in a deep-sea symbiosis. Elife 10:e58371
-**AxioCam MRm3
+= Affiliation, Contact & Terms of use =
+{| border="2"
+|style="width:200px; text-align:left;" |Affiliation:     ||style="width:300px; text-align:left;" |
+:XXXX
+|-
+|Location: ||
+:XXXX
+|-
+|Terms of use: ||
+:XXXX
+|-
+|Biosafety level: ||
+:S2
+|}