Axio Observer.Z1: Unterschied zwischen den Versionen

Aus Imaging Techniken
Die Seite wurde neu angelegt: „= Type of device = Fluorescence microscope by Zeiss <br />Axio Observer with Incubation Chamber = Short description = Live Cell Imaging / Fluorescence Microscope / Brightfield = Instrument parameters = {| border="2" |style="width:200px; text-align:left;" |Technical specifications: ||style="width:500px; text-align:left;" | :Upright system :Motorized stage :z-stacking :HPX 120 C light source |- |Objectives: || |- |Fluorescence Filter Sets: || :BF,…“
 
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(7 dazwischenliegende Versionen desselben Benutzers werden nicht angezeigt)
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Fluorescence microscope by Zeiss
Fluorescence microscope by Zeiss
<br />Axio Observer with Incubation Chamber
<br />Axio Observer with Incubation Chamber


= Short description =
= Short description =
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= Instrument parameters =
= Instrument parameters =
{| border="2"
{| border="2"
|style="width:200px; text-align:left;" |Technical specifications:    ||style="width:500px; text-align:left;" |
|style="width:200px; text-align:left;" |Technical specifications:    ||style="width:700px; text-align:left;" |
:Upright system
:Upright system
:Motorized stage
:Motorized stage
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:HPX 120 C light source
:HPX 120 C light source
|-
|-
|Objectives: ||
|Flourescent filter sets: ||  
|-
|Fluorescence Filter Sets: ||  
:BF, PH1, DICII, DICIII, DAPI, Cy5 (Far Red), GFP, YFP, Texas Red, CFP, Cy7
:BF, PH1, DICII, DICIII, DAPI, Cy5 (Far Red), GFP, YFP, Texas Red, CFP, Cy7
|-
|-
|Software: ||  
|Software: ||  
:Zen
:Zen 2011
|}
 
 
{| class="wikitable" style="margin:0"
|+ style="text-align:left;" |<span style="font-variant:small-caps">Objectives</span>
|-
! style="width:200px; text-align:left;" |Mag !! style="width:100px; text-align:left;" |NA !! style="width:100px; text-align:left;" |Oil? !! style="width:100px; text-align:left;" |DIC?
|-
| 5x || 0.16 || No || No
|-
| 10x || 0.30 || No || Ph1
|-
| 20x || 0.8 || No || DICI
|-
|40x || 0.75 || No || DICII
|-
|63x EC Plan-Neofluar || 1.4 || Yes || DICII
|}
|}
{| class="wikitable" style="margin:0"
|+ style="text-align:left;" |<span style="font-variant:small-caps">Fluorescence Filter Sets</span>
|-
! style="width:200px; text-align:left;" |Filterset !! style="width:100px; text-align:left;" |Excitation !! style="width:100px; text-align:left;" |Beamsplitter !!style="width:100px; text-align:left;" | Emission
|-
| 43 HE (DsRed) || 550/25 || 570 || 605/70
|-
| 38 HE (GFP) || 470/40 || 495 || 525/50
|-
| 49 DAPI || 359/48 || 395 || 445/50
|}




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= Affiliation, Contact & Terms of use =
= Affiliation, Contact & Terms of use =
{| border="2"
{| border="2"
|style="width:200px; text-align:left;" |Affiliation:    ||style="width:500px; text-align:left;" |
|style="width:200px; text-align:left;" |Affiliation:    ||style="width:700px; text-align:left;" |
:XXXX
:XXXX
|-
|-

Aktuelle Version vom 6. Mai 2025, 11:01 Uhr

Type of device

Fluorescence microscope by Zeiss
Axio Observer with Incubation Chamber


Short description

Live Cell Imaging / Fluorescence Microscope / Brightfield


Instrument parameters

Technical specifications:
Upright system
Motorized stage
z-stacking
HPX 120 C light source
Flourescent filter sets:
BF, PH1, DICII, DICIII, DAPI, Cy5 (Far Red), GFP, YFP, Texas Red, CFP, Cy7
Software:
Zen 2011


Objectives
Mag  NA  Oil? DIC?
5x 0.16 No No
10x 0.30 No Ph1
20x 0.8 No DICI
40x 0.75 No DICII
63x EC Plan-Neofluar 1.4 Yes DICII


Fluorescence Filter Sets
Filterset  Excitation  Beamsplitter Emission
43 HE (DsRed) 550/25 570 605/70
38 HE (GFP) 470/40 495 525/50
49 DAPI 359/48 395 445/50



Methods & Expertise

Scratch Assay, Sprout Assay, Tube Formation Assay, fluorescence microscopy, live cell imaging


Reference publications

Heusler M, Einenkel R, Ehrhardt J, Muzzio DO, Zygmunt M. Low Abundance Fusobacterium Nucleatum Supports Early Pregnancy Development - An In Vitro Study. Front Immunol. 2021 Aug 31;12:698045. doi: 10.3389/fimmu.2021.698045. PMID: 34531854; PMCID: PMC8438310.

Light Sci Appl. 2022 Sep 7;11(1):265. doi: 10.1038/s41377-022-00952-z. GANscan: continuous scanning microscopy using deep learning deblurring Michael John Fanous 1 2, Gabriel Popescu 3 4 5 Affiliations Expand PMID: 36071043 PMCID: PMC9452654 DOI: 10.1038/s41377-022-00952-z

J Ophthalmol. 2021 Mar 19:2021:6623794. doi: 10.1155/2021/6623794. eCollection 2021. Histopathological Variability and Concomitant Lesions in Pterygium in a Large Case Series Sabrina Bergeron 1, Hiroaki Ito 1 2, Yves E Dossous 1, Miguel N Burnier Jr 1 3 Affiliations Expand PMID: 33828861 PMCID: PMC8004360 DOI: 10.1155/2021/6623794

Diagn Pathol. 2015 Jul 25:10:119. doi: 10.1186/s13000-015-0343-8. Diagnostic imaging of cervical intraepithelial neoplasia based on hematoxylin and eosin fluorescence Mario R Castellanos 1, Anita Szerszen 2, Stephen Gundry 3, Edyta C Pirog 4, Mitchell Maiman 5, Sritha Rajupet 6, John Paul Gomez 6, Adi Davidov 5, Priya Ranjan Debata 7, Probal Banerjee 7, Jimmie E Fata 8 Affiliations Expand PMID: 26204927 PMCID: PMC4513699 DOI: 10.1186/s13000-015-0343-8



Affiliation, Contact & Terms of use

Affiliation:
XXXX
Location:
XXXX
Contact person:
XXXX
Terms of use:
XXXX
Biosafety level:
S1
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