AxioImager.M2: Unterschied zwischen den Versionen

Aus Imaging Techniken
Keine Bearbeitungszusammenfassung
Keine Bearbeitungszusammenfassung
 
(16 dazwischenliegende Versionen desselben Benutzers werden nicht angezeigt)
Zeile 1: Zeile 1:
= Type of device =
= Type of device =
*Fluorescence microscope by Zeiss
Fluorescence microscope by Zeiss




= Short description =
= Short description =
* Modes:
Modes:
**Bright field
*Bright field
**Fluorescence (DAPI, GFP, RFP, CFP, Cy5)
*Fluorescence (DAPI, GFP, RFP, CFP, Cy5)
 


= Instrument parameters =
= Instrument parameters =
{| border="1"
{| border="2"
|Technical specifications
|style="width:200px; text-align:left;" |Technical specifications:    ||style="width:500px; text-align:left;" |
|Äpfel
:Upright system
:Motorized stage
:z-stacking
:HPX 120 C light source
|-
|-
|*Upright system, *Motorized stage, z-stacking
|Objectives: ||
|Kuchen
:EC PlnN 10x/0.3 Ph1 DICI
:EC PlnN 40x/0.75 Ph2
:EC PlnN 100x/1,3 Oil Ph3
|-
|-
|Butter
|Camera: ||
|Eiskrem
:AxioCam MRm3
|-
|Software: ||
:Zen 2011
|}
|}
= Methods & Expertise =
Fluorescence ''in situ'' hybridization
= Reference publications =
*Petersen, I., Schlüter, R., Hoff, K.J., Liebscher, V., Bange, G., Riedel, K. & Pané-Farré, J. (2020) Non-invasive and label-free 3D-visualization shows ''in vivo'' oligomerization of the staphylococcal alkaline shock protein 23 (Asp23). Sci Rep. 10(1):125.
*Hinzke, T., Kleiner, M., Meister, M., Schlüter, R., Hentschker, C., Pané-Farré, J., Hildebrandt, P., Felbeck, H., Sievert, S.M., Bonn, F., Völker, U., Becher, D., Schweder, T. & Markert, S. (2021) Bacterial symbiont subpopulations have different roles in a deep-sea symbiosis. Elife 10:e58371






*Technical specifications:  
= Affiliation, Contact & Terms of use =
**Upright system
{| border="2"
**Motorized stage, z-stacking
|style="width:200px; text-align:left;" |Affiliation:     ||style="width:500px; text-align:left;" |
**HPX 120 C light source
:University of Greifswald, Imaging Center of the Department of Biology
|-
|Location: ||
:Institute of Microbiology
:Felix-Hausdorff-Str. 8


*Objectives:
|-
**EC PlnN 10x/0.3 Ph1 DICI
|Contact person: ||
**EC PlnN 40x/0.75 Ph2
:Dr. Daniela Zühlke
**EC PlnN 100x/1,3 Oil Ph3
:zuehlke@uni-greifswald.de | Phone: 03834 420 5912


*Camera:
|-
**AxioCam MRm3
|Terms of use: ||
:https://imaging.uni-greifswald.de/nutzungsordnung/nutzungsordnungen/fluoreszenzmikroskope-am-institut-fuer-mikrobiologie/
|-
|Biosafety level: ||
:S2
|}

Aktuelle Version vom 4. Februar 2026, 14:44 Uhr

Type of device

Fluorescence microscope by Zeiss


Short description

Modes:

  • Bright field
  • Fluorescence (DAPI, GFP, RFP, CFP, Cy5)


Instrument parameters

Technical specifications:
Upright system
Motorized stage
z-stacking
HPX 120 C light source
Objectives:
EC PlnN 10x/0.3 Ph1 DICI
EC PlnN 40x/0.75 Ph2
EC PlnN 100x/1,3 Oil Ph3
Camera:
AxioCam MRm3
Software:
Zen 2011


Methods & Expertise

Fluorescence in situ hybridization


Reference publications

  • Petersen, I., Schlüter, R., Hoff, K.J., Liebscher, V., Bange, G., Riedel, K. & Pané-Farré, J. (2020) Non-invasive and label-free 3D-visualization shows in vivo oligomerization of the staphylococcal alkaline shock protein 23 (Asp23). Sci Rep. 10(1):125.
  • Hinzke, T., Kleiner, M., Meister, M., Schlüter, R., Hentschker, C., Pané-Farré, J., Hildebrandt, P., Felbeck, H., Sievert, S.M., Bonn, F., Völker, U., Becher, D., Schweder, T. & Markert, S. (2021) Bacterial symbiont subpopulations have different roles in a deep-sea symbiosis. Elife 10:e58371


Affiliation, Contact & Terms of use

Affiliation:
University of Greifswald, Imaging Center of the Department of Biology
Location:
Institute of Microbiology
Felix-Hausdorff-Str. 8
Contact person:
Dr. Daniela Zühlke
zuehlke@uni-greifswald.de | Phone: 03834 420 5912
Terms of use:
https://imaging.uni-greifswald.de/nutzungsordnung/nutzungsordnungen/fluoreszenzmikroskope-am-institut-fuer-mikrobiologie/
Biosafety level:
S2