Operetta CLS - Versionsgeschichte

Admin: /* Affiliation, Contact & Terms of use */

2026-01-30T09:46:19Z

Affiliation, Contact & Terms of use

← Nächstältere Version		Version vom 30. Januar 2026, 09:46 Uhr
Zeile 131:		Zeile 131:
	\|Contact person: \|\|		\|Contact person: \|\|
	:Prof. Dr. Sander Bekeschus		:Prof. Dr. Sander Bekeschus
	:~~sander.bekeschus@gmail.com~~		:
	\|-		\|-
	\|Terms of use: \|\|		\|Terms of use: \|\|

Admin: /* Affiliation, Contact & Terms of use */

2026-01-26T10:14:34Z

Affiliation, Contact & Terms of use

← Nächstältere Version		Version vom 26. Januar 2026, 10:14 Uhr
Zeile 120:		Zeile 120:
	{\| border="2"		{\| border="2"
	\|style="width:200px; text-align:left;" \|Affiliation: \|\|style="width:500px; text-align:left;" \|		\|style="width:200px; text-align:left;" \|Affiliation: \|\|style="width:500px; text-align:left;" \|
	:~~XXXX~~		:Leibniz Institute for Plasma Science and Technology (INP), Greifswald
	\|-		\|-
	\|Location: \|\|		\|Location: \|\|
	:~~XXXX~~		:Leibniz Institute for Plasma Science and Technology (INP), Greifswald
			:ZIK plasmatis
			:Felix-Hausdorff-Str. 2
			:17489 Greifswald

	\|-		\|-
	\|Contact person: \|\|		\|Contact person: \|\|
	:~~XXXX~~		:Prof. Dr. Sander Bekeschus
			:sander.bekeschus@gmail.com
	\|-		\|-
	\|Terms of use: \|\|		\|Terms of use: \|\|
	:~~XXXX~~		:*Imaging and data analysis only in collaboration with AG Bekeschus
			:*Image acquisition only onto INP servers into the connected data base (but selected images can be exported and saved on drives from INP computers)

	\|-		\|-
	\|Biosafety level: \|\|		\|Biosafety level: \|\|
	:None/S2		:None/S2
	\|}		\|}

Admin am 7. Mai 2025 um 10:59 Uhr

2025-05-07T10:59:56Z

@@ Zeile 26: / Zeile 26: @@
 |Objectives: ||
 :3 air and 3 water-emmersion objectives permanently installed
-:All objectives in the following table except 20x hNA air and 40x hNA air
+:All objectives in the table below
 |-
 |Laser: ||
@@ Zeile 34: / Zeile 34: @@
 :Harmony 4.9
 |}

Admin am 6. Mai 2025 um 11:18 Uhr

2025-05-06T11:18:33Z

← Nächstältere Version		Version vom 6. Mai 2025, 11:18 Uhr
Zeile 39:		Zeile 39:
	\|+ style="text-align:left;" \|<span style="font-variant:small-caps">Laser: 8 LEDs</span>		\|+ style="text-align:left;" \|<span style="font-variant:small-caps">Laser: 8 LEDs</span>
	\|-		\|-
	! style="width:200px; text-align:left;" \|Wavelength !! style="width:150px; text-align:left;" \|Power !! style="width:150px; text-align:left;" \|Excitation Filter !! style="width:~~150px~~; text-align:left;" \|Classification		! style="width:200px; text-align:left;" \|Wavelength !! style="width:150px; text-align:left;" \|Power !! style="width:150px; text-align:left;" \|Excitation Filter !! style="width:170px; text-align:left;" \|Classification
	\|-		\|-
	\| 365 nm (UV) \|\| 130 mW \|\| 355-385 nm \|\| Risk Group 3		\| 365 nm (UV) \|\| 130 mW \|\| 355-385 nm \|\| Risk Group 3
Zeile 62:		Zeile 62:
	\|+ style="text-align:left;" \|<span style="font-variant:small-caps">Autofocus Laser</span>		\|+ style="text-align:left;" \|<span style="font-variant:small-caps">Autofocus Laser</span>
	\|-		\|-
	! style="width:200px; text-align:left;" \|Wavelength !! style="width:150px; text-align:left;" \|Laser Power !! style="width:~~150px~~; text-align:left;" \|Descrption		! style="width:200px; text-align:left;" \|Wavelength !! style="width:150px; text-align:left;" \|Laser Power !! style="width:330px; text-align:left;" \|Descrption
	\|-		\|-
	\| 785+/-10 nm \|\| 12 mW \|\|		\| 785+/-10 nm \|\| 12 mW \|\|
	sCMOS camera		sCMOS camera
	Laser class 3B		<br />Laser class 3B
	\|}		\|}

Zeile 73:		Zeile 73:
	\|+ style="text-align:left;" \|<span style="font-variant:small-caps">Transmission Light Source</span>		\|+ style="text-align:left;" \|<span style="font-variant:small-caps">Transmission Light Source</span>
	\|-		\|-
	! style="width:200px; text-align:left;" \|Wavelength !! style="width:150px; text-align:left;" \|Laser Power !! style="width:~~150px~~; text-align:left;" \|Descrption		! style="width:200px; text-align:left;" \|Wavelength !! style="width:150px; text-align:left;" \|Laser Power !! style="width:330px; text-align:left;" \|Descrption
	\|-		\|-
	\| 740 nm \|\| 80 mW \|\|		\| 740 nm \|\| 80 mW \|\|
	LED for Brightfiled and Digital Phase Contrast measurements		LED for Brightfiled and Digital Phase Contrast measurements
	Integrated in sample lid		<br />Integrated in sample lid
	\|}		\|}

Admin am 6. Mai 2025 um 11:14 Uhr

2025-05-06T11:14:07Z

← Nächstältere Version		Version vom 6. Mai 2025, 11:14 Uhr
Zeile 34:		Zeile 34:
	:Harmony 4.9		:Harmony 4.9
	\|}		\|}


			{\| class="wikitable" style="margin:0"
			\|+ style="text-align:left;" \|<span style="font-variant:small-caps">Laser: 8 LEDs</span>
			\|-
			! style="width:200px; text-align:left;" \|Wavelength !! style="width:150px; text-align:left;" \|Power !! style="width:150px; text-align:left;" \|Excitation Filter !! style="width:150px; text-align:left;" \|Classification
			\|-
			\| 365 nm (UV) \|\| 130 mW \|\| 355-385 nm \|\| Risk Group 3
			\|-
			\| 405 nm \|\| 230 mW \|\| 390-420 nm \|\| Risk Group 3
			\|-
			\| 450 nm \|\| 230 mW \|\| 435-460 nm \|\| Risk Group 3
			\|-
			\| 475 nm \|\| 110 mW \|\| 460-490 nm \|\| Risk Group 2
			\|-
			\| 505 nm \|\| 100 mW \|\| 490-515 nm \|\| Risk Group 2
			\|-
			\| 550 nm \|\| 170 mW \|\| 530-560 nm \|\| Risk Group 2
			\|-
			\| 630 nm \|\| 120 mW \|\| 615-645 nm \|\| Risk Group 2
			\|-
			\| 660 nm \|\| 250 mW \|\| 650-675 nm \|\| Risk Group 3
			\|}


			{\| class="wikitable" style="margin:0"
			\|+ style="text-align:left;" \|<span style="font-variant:small-caps">Autofocus Laser</span>
			\|-
			! style="width:200px; text-align:left;" \|Wavelength !! style="width:150px; text-align:left;" \|Laser Power !! style="width:150px; text-align:left;" \|Descrption
			\|-
			\| 785+/-10 nm \|\| 12 mW \|\|
			sCMOS camera
			Laser class 3B
			\|}


			{\| class="wikitable" style="margin:0"
			\|+ style="text-align:left;" \|<span style="font-variant:small-caps">Transmission Light Source</span>
			\|-
			! style="width:200px; text-align:left;" \|Wavelength !! style="width:150px; text-align:left;" \|Laser Power !! style="width:150px; text-align:left;" \|Descrption
			\|-
			\| 740 nm \|\| 80 mW \|\|
			LED for Brightfiled and Digital Phase Contrast measurements
			Integrated in sample lid
			\|}

Admin: Schützte „Operetta CLS“ ([Bearbeiten=Nur automatisch bestätigten Benutzern erlauben] (unbeschränkt) [Verschieben=Nur automatisch bestätigten Benutzern erlauben] (unbeschränkt))

2025-04-30T05:36:42Z

Schützte „Operetta CLS“ ([Bearbeiten=Nur automatisch bestätigten Benutzern erlauben] (unbeschränkt) [Verschieben=Nur automatisch bestätigten Benutzern erlauben] (unbeschränkt))

← Nächstältere Version	Version vom 30. April 2025, 05:36 Uhr
(kein Unterschied)

Admin: Die Seite wurde neu angelegt: „= Type of device = High-Content Imager / Confocal Scanning Microscopy / Live-Cell Imaging
Operetta CLS by PerkinElmer (Revity) = Short description = The device is flexible for different imaging situations that require quantification: Well plate plate types and slides Automated re-imaging, e.g., re-image cells with 10% largest nuclei at higher magnification Widefield and spinning-disk confocal imaging Live-cell imaging (37°C, CO₂ v…“

2025-04-29T13:11:46Z

Die Seite wurde neu angelegt: „= Type of device = High-Content Imager / Confocal Scanning Microscopy / Live-Cell Imaging Operetta CLS by PerkinElmer (Revity) = Short description = The device is flexible for different imaging situations that require quantification: *Well plate plate types and slides *Automated re-imaging, e.g., re-image cells with 10% largest nuclei at higher magnification *Widefield and spinning-disk confocal imaging *Live-cell imaging (37°C, CO2 v…“

Neue Seite

= Type of device =
High-Content Imager / Confocal Scanning Microscopy / Live-Cell Imaging
 Operetta CLS by PerkinElmer (Revity)

= Short description =
The device is flexible for different imaging situations that require quantification:
*Well plate plate types and slides
*Automated re-imaging, e.g., re-image cells with 10% largest nuclei at higher magnification
*Widefield and spinning-disk confocal imaging
*Live-cell imaging (37°C, CO2 variable) over time also with water-objectives (autom. refill)

= Instrument parameters =
{| border="2"
|style="width:200px; text-align:left;" |Technical specifications: ||style="width:500px; text-align:left;" |
:sCMOS camera
:Motorized stage
:Confocal Disk
:Transmitted light and digital phase contrast
:Ratiometric FRET
:3D-imaging and live cell imaging
:Fluorescence, fluorescence transmittance, transmission
|-
|Objectives: ||
:3 air and 3 water-emmersion objectives permanently installed
:All objectives in the following table except 20x hNA air and 40x hNA air
|-
|Laser: ||
:8 LEDs
|-
|Software: ||
:Harmony 4.9
|}

= Methods & Expertise =
Many different imaging assays and quantifications, e.g., migration assay, 3D spheroid growth tracking, organoid imaging and three-dimensional object segmentation and quantification, zebrafish larvae imaging, actin length quantification, scratch assays, single-cell sorting verification (single cell per well) > all in 96-well plates or similar; whole tissue section imaging on up to four slides, prescan-rescan assays for automated re-imaging of specific sub-populations within cell lines and primary cells

= Reference publications =
Over 10 publications together with University (Medicine) Greifswald, e.g., on human organoids (DOI: 10.1007/s00018-022-04355-6 Fig. 4e)
 Over 30 publications in house, e.g., on high-throughput micronucleus analysis (DOI: 10.3390/nano12244463 Fig. 1), actin protrusion quantification (DOI: 10.1038/s41598-018-37169-3 Fig. 5), and whole-tissue imaging and quantification (DOI: 10.1080/2162402X.2024.2425124 Fig. 1-3)

= Affiliation, Contact & Terms of use =
{| border="2"
|style="width:200px; text-align:left;" |Affiliation: ||style="width:500px; text-align:left;" |
:XXXX
|-
|Location: ||
:XXXX
|-
|Contact person: ||
:XXXX
|-
|Terms of use: ||
:XXXX
|-
|Biosafety level: ||
:None/S2
|}