Axio Observer.Z1

2026-01-27T11:37:47Z

Admin: /* Reference publications */

Axio Observer.Z1

2026-01-27T11:37:38Z

Admin: /* Instrument parameters */

Datei:Nutzungsbedingungen Wiki GIRT.pdf

2026-01-27T08:49:30Z

Admin: Schützte „Datei:Nutzungsbedingungen Wiki GIRT.pdf“ ([Bearbeiten=Nur Administratoren erlauben] (unbeschränkt) [Verschieben=Nur Administratoren erlauben] (unbeschränkt) [Hochladen=Nur Administratoren erlauben] (unbeschränkt))

Datei:Datenschutzhinweise Wiki GIRT.pdf

2026-01-27T08:49:26Z

Admin: Schützte „Datei:Datenschutzhinweise Wiki GIRT.pdf“ ([Bearbeiten=Nur Administratoren erlauben] (unbeschränkt) [Verschieben=Nur Administratoren erlauben] (unbeschränkt) [Hochladen=Nur Administratoren erlauben] (unbeschränkt))

Datei:Terms-of-use Wiki GIRT.pdf

2026-01-27T08:49:22Z

Admin: Schützte „Datei:Terms-of-use Wiki GIRT.pdf“ ([Bearbeiten=Nur Administratoren erlauben] (unbeschränkt) [Verschieben=Nur Administratoren erlauben] (unbeschränkt) [Hochladen=Nur Administratoren erlauben] (unbeschränkt))

2026-01-26T15:24:18Z

Admin:

=MediaWiki zum GIRT=

Im Rahmen des Greifswald Imaging Round Table (GIRT) können die Projektteilnehmenden Informationen über dieses MediaWiki austauschen.

Sie möchten einen Login beantragen? 
Dann schreiben Sie eine Nachricht an wiki-imaging@uni-greifswald.de und erhalten Zugriff auf das Wiki.

HINWEIS: Mit Beantragung des Logins willigen Sie in die Verarbeitung Ihrer personenbezogenen Daten gemäß den [[Medium:Datenschutzhinweise Wiki GIRT.pdf|Datenschutzhinweisen]] ein und stimmen den [[Medium:Nutzungsbedingungen Wiki GIRT.pdf|Nutzungsbedingungen]] für das Wiki zu. 

<div style="border-top: 2px dashed #000; margin: 1em 0;"></div> 

=MediaWiki for the GIRT=

Project participants of the Greifswald Imaging Round Table (GIRT) can exchange information via this MediaWiki.

Would you like to request a login? 
Then write us an email to wiki-imaging@uni-greifswald.de to receive access to the wiki.

PLEASE NOTE: By requesting a login, you grant consent for the processing of your personal data pursuant to the [[Medium:Information-on-Data-Processing Wiki GIRT.pdf|Data Protection Information]] and accept the wiki’s Terms of Use.

== Starthilfen ==

* Hilfe zur Verwendung und Konfiguration der Wiki-Software findest du im [https://www.mediawiki.org/wiki/Special:MyLanguage/Help:Contents Benutzerhandbuch].
* [https://www.mediawiki.org/wiki/Special:MyLanguage/Manual:Configuration_settings Liste der Konfigurationsparameter]
* [https://www.mediawiki.org/wiki/Special:MyLanguage/Manual:FAQ Häufige Fragen zu MediaWiki]
* [https://lists.wikimedia.org/postorius/lists/mediawiki-announce.lists.wikimedia.org/ Mailingliste zu neuen Versionen von MediaWiki]
* [https://www.mediawiki.org/wiki/Special:MyLanguage/Localisation#Translation_resources Übersetze MediaWiki für deine Sprache]
* [https://www.mediawiki.org/wiki/Special:MyLanguage/Manual:Combating_spam Erfahre, wie du Spam auf deinem Wiki bekämpfen kannst]

Datei:Information-on-Data-Processing Wiki-GIRT.pdf

2026-01-26T15:21:59Z

Admin:

Datei:Terms-of-use Wiki GIRT.pdf

2026-01-26T15:21:34Z

Admin:

Hauptseite

2026-01-26T15:20:53Z

Admin:

=MediaWiki zum GIRT=

Im Rahmen des Greifswald Imaging Round Table (GIRT) können die Projektteilnehmenden Informationen über dieses MediaWiki austauschen.

Sie möchten einen Login beantragen? 
Dann schreiben Sie eine Nachricht an wiki-imaging@uni-greifswald.de und erhalten Zugriff auf das Wiki.

HINWEIS: Mit Beantragung des Logins willigen Sie in die Verarbeitung Ihrer personenbezogenen Daten gemäß den [[Medium:Datenschutzhinweise Wiki GIRT.pdf|Datenschutzhinweisen]] ein und stimmen den [[Medium:Nutzungsbedingungen Wiki GIRT.pdf|Nutzungsbedingungen]] für das Wiki zu. 

<div style="border-top: 2px dashed #000; margin: 1em 0;"></div> 

=MediaWiki for the GIRT=

Project participants of the Greifswald Imaging Round Table (GIRT) can exchange information via this MediaWiki.

Would you like to request a login? 
Then write us an email to wiki-imaging@uni-greifswald.de to receive access to the wiki.

PLEASE NOTE: By requesting a login, you grant consent for the processing of your personal data pursuant to the Data Protection Information and accept the wiki’s Terms of Use.

== Starthilfen ==

* Hilfe zur Verwendung und Konfiguration der Wiki-Software findest du im [https://www.mediawiki.org/wiki/Special:MyLanguage/Help:Contents Benutzerhandbuch].
* [https://www.mediawiki.org/wiki/Special:MyLanguage/Manual:Configuration_settings Liste der Konfigurationsparameter]
* [https://www.mediawiki.org/wiki/Special:MyLanguage/Manual:FAQ Häufige Fragen zu MediaWiki]
* [https://lists.wikimedia.org/postorius/lists/mediawiki-announce.lists.wikimedia.org/ Mailingliste zu neuen Versionen von MediaWiki]
* [https://www.mediawiki.org/wiki/Special:MyLanguage/Localisation#Translation_resources Übersetze MediaWiki für deine Sprache]
* [https://www.mediawiki.org/wiki/Special:MyLanguage/Manual:Combating_spam Erfahre, wie du Spam auf deinem Wiki bekämpfen kannst]

2026-01-26T14:57:19Z

Admin:

Operetta CLS

2026-01-26T10:14:34Z

Admin: /* Affiliation, Contact & Terms of use */

= Type of device =
High-Content Imager / Confocal Scanning Microscopy / Live-Cell Imaging
 Operetta CLS by PerkinElmer (Revity)

= Short description =
The device is flexible for different imaging situations that require quantification:
*Well plate plate types and slides
*Automated re-imaging, e.g., re-image cells with 10% largest nuclei at higher magnification
*Widefield and spinning-disk confocal imaging
*Live-cell imaging (37°C, CO2 variable) over time also with water-objectives (autom. refill)

= Instrument parameters =
{| border="2"
|style="width:200px; text-align:left;" |Technical specifications: ||style="width:500px; text-align:left;" |
:sCMOS camera
:Motorized stage
:Confocal Disk
:Transmitted light and digital phase contrast
:Ratiometric FRET
:3D-imaging and live cell imaging
:Fluorescence, fluorescence transmittance, transmission
|-
|Objectives: ||
:3 air and 3 water-emmersion objectives permanently installed
:All objectives in the table below
|-
|Laser: ||
:8 LEDs
|-
|Software: ||
:Harmony 4.9
|}

{| class="wikitable" style="margin:0"
|+ style="text-align:left;" |Objectivs
|-
! style="width:100px; text-align:left;" |Objective !! style="width:80px; text-align:left;" |NA !! style="width:100px; text-align:left;" |Working distance [mm] !! style="width:150px; text-align:left;" |Corrected for plate thickness [mm] !! style="width:120px; text-align:left;" |Field of view [mm] !! style="width:90px; text-align:left;" |Dept of focus [µm]
|-
| 1.25x Air || 0.03 || 4 || 0.17 || 10.33 x 10.33 || 1501.0
|-
| 5x Air || 0.16 || 12.1 || 0.17 || 2.58 x 2.58 || 58.2
|-
| 10x Air || 0.3 || 5.2 || 0.17 || 1.29 x 1.29 || 16.2
|-
| 20x Air || 0.4 || 8.28 || 0 - 1.5 || 0.65 x 0.65 || 8.0
|-
| 20x Water || 1.0 || 1.7 || 0.17 || 0.65 x 0.65 || 1.8
|-
| 40x Air || 0.6 || 3.28 || 0 - 1.5 || 0.32 x 0.32 || 3.3
|-
| 40x Water || 1.1 || 0.62 || 0.17 || 0.32 x 0.32 || 1.2
|-
| 63x Water || 1.15 || 0.6 || 0.17 || 0.21 x 0.21 || 1.0
|}

{| class="wikitable" style="margin:0"
|+ style="text-align:left;" |Laser: 8 LEDs
|-
! style="width:200px; text-align:left;" |Wavelength !! style="width:150px; text-align:left;" |Power !! style="width:150px; text-align:left;" |Excitation Filter !! style="width:170px; text-align:left;" |Classification
|-
| 365 nm (UV) || 130 mW || 355-385 nm || Risk Group 3
|-
| 405 nm || 230 mW || 390-420 nm || Risk Group 3
|-
| 450 nm || 230 mW || 435-460 nm || Risk Group 3
|-
| 475 nm || 110 mW || 460-490 nm || Risk Group 2
|-
| 505 nm || 100 mW || 490-515 nm || Risk Group 2
|-
| 550 nm || 170 mW || 530-560 nm || Risk Group 2
|-
| 630 nm || 120 mW || 615-645 nm || Risk Group 2
|-
| 660 nm || 250 mW || 650-675 nm || Risk Group 3
|}

{| class="wikitable" style="margin:0"
|+ style="text-align:left;" |Autofocus Laser
|-
! style="width:200px; text-align:left;" |Wavelength !! style="width:150px; text-align:left;" |Laser Power !! style="width:330px; text-align:left;" |Descrption
|-
| 785+/-10 nm || 12 mW ||
sCMOS camera
 Laser class 3B
|}

{| class="wikitable" style="margin:0"
|+ style="text-align:left;" |Transmission Light Source
|-
! style="width:200px; text-align:left;" |Wavelength !! style="width:150px; text-align:left;" |Laser Power !! style="width:330px; text-align:left;" |Descrption
|-
| 740 nm || 80 mW ||
LED for Brightfiled and Digital Phase Contrast measurements
 Integrated in sample lid
|}

= Methods & Expertise =
Many different imaging assays and quantifications, e.g., migration assay, 3D spheroid growth tracking, organoid imaging and three-dimensional object segmentation and quantification, zebrafish larvae imaging, actin length quantification, scratch assays, single-cell sorting verification (single cell per well) > all in 96-well plates or similar; whole tissue section imaging on up to four slides, prescan-rescan assays for automated re-imaging of specific sub-populations within cell lines and primary cells

= Reference publications =
Over 10 publications together with University (Medicine) Greifswald, e.g., on human organoids (DOI: 10.1007/s00018-022-04355-6 Fig. 4e)
 Over 30 publications in house, e.g., on high-throughput micronucleus analysis (DOI: 10.3390/nano12244463 Fig. 1), actin protrusion quantification (DOI: 10.1038/s41598-018-37169-3 Fig. 5), and whole-tissue imaging and quantification (DOI: 10.1080/2162402X.2024.2425124 Fig. 1-3)

= Affiliation, Contact & Terms of use =
{| border="2"
|style="width:200px; text-align:left;" |Affiliation: ||style="width:500px; text-align:left;" |
:Leibniz Institute for Plasma Science and Technology (INP), Greifswald
|-
|Location: ||
:Leibniz Institute for Plasma Science and Technology (INP), Greifswald
:ZIK plasmatis
:Felix-Hausdorff-Str. 2
:17489 Greifswald

|-
|Contact person: ||
:Prof. Dr. Sander Bekeschus
:sander.bekeschus@gmail.com
|-
|Terms of use: ||
:*Imaging and data analysis only in collaboration with AG Bekeschus
:*Image acquisition only onto INP servers into the connected data base (but selected images can be exported and saved on drives from INP computers)

|-
|Biosafety level: ||
:None/S2
|}

Zeiss LSM980

2026-01-26T10:00:31Z

Admin: /* Affiliation, Contact & Terms of use */

= Type of device =
Confocal laser scanning microscope by Zeiss
 LSM980 with Airyscan 2

= Short description =
The Zeiss LSM980 with Airyscan 2 is optimized for the simultaneous spectral detection of multiple weak labels with highest light efficiency. It offers excellent imaging performance combined with low phototoxicity and high speed. It can be used for multi-color experiments with living or fixed samples with highest sensitivity and spectral flexibility.

= Instrument parameters =
{| border="2"
|style="width:200px; text-align:left;" |Technical specifications: ||style="width:500px; text-align:left;" |
:Inverse microscope LSM 980
:Incubator XLmulti S1 with temperature control
:Optional lid for CO2 and O2 control
:z-piezo
:LED Colibri 7
:Definite Focus 2
:T-PMT detector for transmission
:Airyscan 2 with 32 GaAsP-detector elements for Super-resolution imaging
|-
|Objectives: ||
:Plan-Apochromat 10x/0,45 M27
:Plan-Apochromat 20x/0,8 M27
:Plan-Apochromat 40x/0,95 Korr M27
:LD LCI Plan-Apochromat 40x/1,2 Imm Korr DIC M27
:Plan-Apochromat 63x/1,4 Oil DIC M27
|-
|Laser: ||
:Dioden Laser 405 nm
:Dioden Laser 488 nm
:DPSS Laser 561 nm
:Dioden Laser 639 nm
|-
|Software: ||
:ZEN blue
|}

= Methods & Expertise =
Tiles, z-stack, time series, bleaching, FRAP

= Reference publications =
Komaragiri, Y. , Pires, R. H., Spiegler, S, Dau, H. T., Biedenweg, D., Salas, C. O., Hossain, Md. F., Fregin, B., Gross, S., Gellert, M., Lendeckel, U., Lillig, C. & Otto, O. (2024) Reactive oxygen species induce cell stiffening through lysosomal degradation and subsequent intracellular acidosis in myeloid precursor cells. Communications Physics 7:252

= Affiliation, Contact & Terms of use =
{| border="2"
|style="width:200px; text-align:left;" |Affiliation: ||style="width:500px; text-align:left;" |
:Research Group of Cellular Biophysics, Institute of Physics, University of Greifswald
|-
|Location: ||
:University of Greifswald
:Research Group of Cellular Biophysics
:Institute of Physics
:Friedrich-Ludwig-Jahn-Str. 15a
:17489 Greifswald

|-
|Contact person: ||
:Dr. rer. nat. Stefanie Spiegler
: stefanie.spiegler@uni-greifswald.de | Phone: 03834 / 420 5609
|-
|Terms of use: ||
:
|-
|Biosafety level: ||
:S1
|}